Comparison of different sewage sludge pretreatment technologies for improving sludge solubilization and anaerobic digestion efficiency: A comprehensive review.

Anaerobic digestion (AD) of sewage sludge reduces organic solids and produces methane, but the complex nature of sludge, especially the difficulty in solubilization, limits AD efficiency. Pretreatments, by destroying sludge structure and promoting disintegration and hydrolysis, are valuable strategies to enhance AD performance. There is a plethora of reviews on sludge pretreatments, however, quantitative comparisons from multiple perspectives across different pretreatments remain scarce. This review categorized various pretreatments into three groups: Physical (ultrasonic, microwave, thermal hydrolysis, electric decomposition, and high pressure homogenization), chemical (acid, alkali, Fenton, calcium peroxide, and ozone), and biological (microaeration, exogenous bacteria, and exogenous hydrolase) pretreatments. The optimal conditions of various pretreatments and their impacts on enhancing AD efficiency were summarized; the effects of different pretreatments on microbial community in the AD system were comprehensively compared. The quantitative comparison based on dissolution degree of COD (DDCOD) indicted that the sludge solubilization performance is in the order of physical, chemical, and biological pretreatments, although with each below 40 % DDCOD. Biological pretreatment, particularly microaeration and exogenous bacteria, excel in AD enhancement. Pretreatments alter microbial ecology, favoring Firmicutes and Methanosaeta (acetotrophic methanogens) over Proteobacteria and Methanobacterium (hydrogenotrophic methanogens). Most pretreatments have unfavorable energy and economic outcomes, with electric decomposition and microaeration being exceptions. On the basis of the overview of the above pretreatments, a full energy and economy assessment for sewage sludge treatment was suggested. Finally, challenges associated with sludge pretreatments and AD were analyzed, and future research directions were proposed. This review may broaden comprehension of sludge pretreatments and AD, and provide an objective basis for the selection of sludge pretreatment technologies.

Evaluating effects of biochar on anaerobic digestion of dewatered waste activated sludge: Digester performance, microbial co-metabolism and underlying mechanism.

Biochar has been proven to be capable of improving the performance of anaerobic digestion (AD). However, the effect of biochar on microbial communities remains ambiguous. In this study, the influence of pH was excluded in a semi-continuous anaerobic digestor for the treatment of dewatered waste activated sludge (WAS) to determine the effect of biochar on microbes. Compared with the control group, the average methane production increased by 24.5% and 23.2% at the organic loading rates (OLRs) of 1.56 and 3.00 gTS/L/d, respectively, in the presence of biochar. This study innovatively found biochar accelerated the enrichment of Methanofastidiosaceae, which competed with Methanobacteriaceae for H2, and its abundance increased from 0.99% at the OLR of 1.56 g TS/L/d to 16.57% and 38.11% at the OLR of 3.00 and 5.60 gTS/L/d, respectively. The efficient metabolic network of f__norank_o__Aminicenantales, syntrophic bacteria, Methanofastidiosaceae and Methanosaetaceae promoted the conversion of WAS to CH4 in the biochar group. In addition, metagenome analysis revealed that biochar optimized the metabolites related to energy conservation and electron transfer, particularly for hydrogenase (frhABG, mbhLHK and hndA-D), confirming that biochar changed the way H2 was involved in methanogenesis. These findings provide novel insights into the direct effect of biochar on microbial evolution and facilitate the reduction of WAS to achieve higher economic benefits in biogas production.

A novel approach for the fractionation of organic components and microbial degraders in ADM1 and model validation based on the methanogenic potential.

The anaerobic digestion model No 1 (ADM1), with fixed fractions of the substrate components, is currently used to simulate methane production during the anaerobic digestion (AD) of waste activated sludge (WAS). However, the goodness-of-fit for the simulation is not ideal due to the different characteristics of WAS from different regions. In this study, a novel methodology based on a modern instrumental analysis and 16S rRNA gene sequence analysis for the fractionation of organic components and microbial degraders in the WAS is investigated to modify the fractions of the components in the ADM1. The combination of Fourier transform infrared (FTIR), X-ray photoelectron spectroscopy (XPS), and nuclear magnetic resonance (NMR) analyses were used to achieve a rapid and accurate fractionation of the primary organic matters in the WAS that was verified using both the sequential extraction method and the excitation-emission matrix (EEM). The protein, carbohydrate, and lipid contents in the four different sludge samples measured using the above combined instrumental analyses were 25.0 - 50.0%, 2.0 - 10.0%, and 0.9 - 2.3%. The microbial diversity based on 16S rRNA gene sequence analysis was utilized to re-set the initial fractions of the microbial degraders in the ADM1. A batch experiment was utilized to further calibrate the kinetic parameters in the ADM1. Based on the above optimization of the stoichiometric and kinetic parameters, the ADM1 with full parameter modification for WAS (ADM1-FPM) simulated the methane production of the WAS very well with a Theil's inequality coefficient (TIC) of 0.049, which was increased by 89.8% than that of the default ADM1 fit. The proposed approach, with its rapid and reliable performance, demonstrated a strong application potential for the fractionation of organic solid waste and the modification of ADM1, which contributed to a better simulation of methane production during the AD of organic solid wastes.

Comprehensive insights into the impact of pretreatment on anaerobic digestion of waste active sludge from perspectives of organic matter composition, thermodynamics, and multi-omics.

Although various pretreatments have been applied to promote the anaerobic digestion of waste active sludge (WAS), the mechanisms regarding the impact of pretreatment on anaerobic digestion have not been well addressed. In this study, the effects of acid, alkali, and thermal pretreatments on anaerobic digestion of WAS were comprehensively investigated from the perspectives of organic matter composition, thermodynamics, and multi-omics. Results showed acid, alkali, and thermal pretreatments increased the methane production potential of WAS by 53.7%, 98.2%, and 101.8%, respectively, compared with the control. The protein secondary structure was disrupted after pretreatment, with a shift from α-helix and ß-sheet to random coil and antiparallel ß-sheet/aggregated strands. Thermodynamically, the WAS flocculation process was controlled by the short-range interfacial interactions described by the extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory, which was positively correlated (R = 0.97, p < 0.05) with the organic matter solubilization of the WAS. After pretreatment, the flocculation energy barrier of pretreated WAS was 4.1 (acid), 7.0 (alkali) and 7.1 (thermal) times higher than that of the control group, respectively. Multi-omics analysis confirmed that pretreatment promoted amino acids (tryptophan, tyrosine, phenylalanine, aspartate, glutamate) metabolism, energy metabolism (ABC transporters) and vitamin metabolism. Moreover, the comparison of upregulated differentially expressed proteins (DEPs) revealed that for amino acid metabolism, thermal treatment had the best promotion effect; for carbohydrate metabolism, alkali treatment had the best promotion effect; and for lipid metabolism, acid treatment was more advantageous, resulting in different anaerobic digestion efficiencies. This study provides an in-depth understanding of the impact of different pretreatments on WAS anaerobic digestion and has practical implication for the choice of proper pretreatment technology for biosolids.

Enhancement methane fermentation of Enteromorpha prolifera waste by Saccharomyces cerevisiae: batch kinetic investigation, dissolved organic matter characterization, and synergistic mechanism.

With the invasion of green tide, there were millions of tons of Enteromorpha prolifera (Enteromorpha) that need to be disposed of. An efficient microecological system for Enteromorpha fermentation was constructed using Saccharomyces cerevisiae (S. cerevisiae) and granular sludge at mesophilic condition (35 °C). In order to investigate the influence of S. cerevisiae dosage on fermentation, biomethane production and variations in dissolved organic matter (DOM) were investigated. The results indicated that the microecosystem with added S. cerevisiae exhibited improved fermentation capacity. Specifically, biomethane production was improved by 18%, with a maximum methane yield of 331 mL/g VS, and the time required to reach 90% methane yield was reduced by 41%. There were positive linear relationships between S. cerevisiae dosage and the efficiency of hydrolysis, acidogenesis, acetogenesis, and methanogenesis (R2 > 0.9). According to theoretical calculations, there was a positive effect of lower S. cerevisiae dosage (less than 0.93 g/g TS) on biomethane production, and excess dosage (more than 0.93 g/g TS) led to a negative effect due to volatile fatty acid (VFA) accumulation. The excitation-emission matrix (EEM) indicated that the humification index (HIX) and fulvic acid (FA) percentage of fluorescence regional integration in the system were decreased because the quinone and ketone groups of the FA accepted electrons from S. cerevisiae. These findings suggested that this microecosystem can accelerate fermentation speed (41%) and increase biomethane output (18.2%). The synergistic effect of Enteromorpha fermentation with Saccharomyces cerevisiae addition.

Accelerated bio-methane production rate in thermophilic digestion of cardboard with appropriate biochar: Dose-response kinetic assays, hybrid synergistic mechanism, and microbial networks analysis.

The effect of biochar on the thermophilic digestion of mono-cardboard was investigated. Compared with control group (T0), the maximum rate of biomethane production was significantly improved after the addition of biochar, especially, it has been improved by 40.6% in T1 (0.77 g/gTS sludge) with the methane production of 89.28 mL/gVS. Also, the addition of biochar improved the efficiency of acidogenesis and acetogenesis. However, adverse effects were observed with the biomethane production decreased by 33.98% and the lag phase extended by 35 h in T5 (3.86 g/gTS sludge). Especially, the results showed that the adsorption of biochar played important roles in digestion. In addition, acetoclastic Methanosaeta which considered to be involved in interspecific electron transfer (IET) was enriched after biochar added and the highest diversity of acetogens was obtained in T1. Oppositely, microbial networks analysis showed that the excessive biochar may destroy the diversity of microorganism drastically.

Revealing the correlation of biomethane generation, DOM fluorescence, and microbial community in the mesophilic co-digestion of chicken manure and sheep manure at different mixture ratio.

Batch co-digestion tests of chicken manure (CM) and sheep manure (SM) at different ratio (Rs/c) were conducted under mesophilic condition (35 °C). Batch kinetic analysis of bioCH4 production, excitation-emission matrix (EEM) fluorescence of dissolved organic matter (DOM), and microbial community were investigated. The well-fitted modified Gompertz model (R2, 0.98-0.99) resulted that the co-digestion markedly improved the methane production rate and shortened the lag phase time. The highest bioCH4 yield of 219.67 mL/gVSadd and maximum production rate of 0.378 mL/gVSadd/h were obtained at an optimum Rs/c of 0.4. Additionally, a significant variation of DOM was detected at the Rs/c of 0.4 with a consistent degradation of soluble microbial byproduct-like and protein-like organics. The positive synergy effects of co-digestion conspicuously enhanced the bioCH4 production efficiency. FI370 and NADH were significantly correlated to Rs/c (p < 0.05). Moreover, the correlations among process indicator, EEM-peaks and different environmental parameters were evaluated by Pearson correlation analysis. The high diversity of acetoclastic methanogens and hydrogenotrophic methanogens in the co-digestion improved the stability of process. Graphical Abstract.

Optimizing biomethane production of mesophilic chicken manure and sheep manure digestion: Mono-digestion and co-digestion kinetic investigation, autofluorescence analysis and microbial community assessment.

Optimization of mesophilic methane production from Chicken manure (CM) and Sheep manure (SM) at total solid (TS) of 8% and 1.6% were obtained by sequence tests in mono-digestion. However, the positive synergy of co-digestion with an optimum CM/SM of 2.5 (310 mLCH4/gVSadded) resulted in a high hydrolytic capacity and methane production. The modified Gompertz model (R2 > 0.98) and modified Aiba model (R2 > 0.88) illustrated co-digestion significantly improved the methane generation rate with strong ammonia tolerance. Dissolved Organic Matter (DOM) variation in response to the metabolic rate of microbial community illustrated that the SMP-like and protein-like components half-split by EEM-PARAFAC were significantly negative corresponded to bio-methane production. Moreover, the canonical correlation analysis (CCA) resulted a significant difference between the substrate and DOM composition. Potential functional metabolic illustrated statistically significance difference between mono and co-digestion, however, Methanosaeta and Syntrophobacter predominated the syntrophic methanogenesis. The constructed complex metabolic cooperation caused the co-digestion stable and high efficiency.

The auto fluorescence characteristics, specific activity, and microbial community structure in batch tests of mono-chicken manure digestion.

Batch tests inoculated with granular and suspended sludge of mono chicken manure (CM) digestion were conducted. Kinetic analysis showed a maximum bio-CH4 generation (6 mL/gVS/d) at an optimal TS of 10-12%. At a TS of 25%, serious inhibition was found for granular sludge and even greater inhibition for the suspended sludge caused by free ammonia. The auto fluorescence of Excitation-mission matrix with parallel factor analysis (PARAFAC) showed that the dissolved organic matter (DOM) varied between the form C1, C2, C3 and C4. The split component of the SMP-like C2 and protein-like C4 significantly related to the bio-methane production in time series. The canonical correlation analysis (CCA) indicated that ammonia, pH, and TS influenced the PARAFAC component significantly. The aceticlastic methanogens of the genus Methanosaeta and acetogens of the genus Syntrophobacter predominated in the CM sludge. The methanogens and acetogens formed a metabolic cooperation, making the process a stable methane produced activity.